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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
22/01/2020 |
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Actualizado : |
24/02/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
RODRIGO, M.J.; LADO, J.; ALÓS, E.; ALQUÉZAR, B.; DERY, O.; HIRSCHBERG, J.; ZACARÍAS, L. |
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Afiliación : |
MARÍA JESÚS RODRIGO, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Spain; JOANNA LADO LINDNER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Spain; ENRIQUETA ALÓS, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Spain; BERTA ALQUÉZAR, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Spain; Instituto de Biología Molecular y Celular de Plantas (IBMCP), UPV-CSIC, Spain; ORLY DERY, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, Israel; JOSEPH HIRSCHBERG, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, Israel; LORENZO ZACARÍAS, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Spain. |
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Título : |
A mutant allele of ζ-carotene isomerase (Z-ISO) is associated with the yellow pigmentation of the "pinalate" sweet orange mutant and reveals new insights into its role in fruit carotenogenesis. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
BMC Plant Biology, 4 November 2019, Volume 19, Issue 1, Article number 465. OPEN ACCESS. Doi: DOI: 10.1186/s12870-019-2078-2 |
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ISSN : |
1471-2229 |
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DOI : |
10.1186/s12870-019-2078-2 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 17 June 2019 / Accepted: 16 October 2019 / Published online: 4 November 2019.
Funding text: Financial support of the research grants AGL2012?34576 and AGL2015? 70218 (Ministerio Economía y Competitividad, Spain), and RTI2018-095131-BI00 (Ministerio Ciencia, Innovacion y Universidades, Spain). Work in the group of JH was supported by Israel Science Foundation Grant 850/13. MJR, LZ, JH and OD are members of Eurocaroten (COST_Action CA15136). MJR and LZ belong to CaRed (Spanish Carotenoid Network BIO2017?90877-REDT, Minis-terio de Ciencia, Innovación y Universidades, Spain). Authors acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI).
Supplementary information accompanies this paper. |
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Contenido : |
Abstract.
Background: Fruit coloration is one of the main quality parameters of Citrus fruit primarily determined by genetic factors. The fruit of ordinary sweet orange (Citrus sinensis) displays a pleasant orange tint due to accumulation of carotenoids, representing β,β-xanthophylls more than 80% of the total content. 'Pinalate' is a spontaneous bud mutant, or somatic mutation, derived from sweet orange 'Navelate', characterized by yellow fruits due to elevated proportions of upstream carotenes and reduced β,β-xanthophylls, which suggests a biosynthetic blockage at early steps of the carotenoid pathway. Results: To identify the molecular basis of 'Pinalate' yellow fruit, a complete characterization of carotenoids profile together with transcriptional changes in carotenoid biosynthetic genes were performed in mutant and parental fruits during development and ripening. 'Pinalate' fruit showed a distinctive carotenoid profile at all ripening stages, accumulating phytoene, phytofluene and unusual proportions of 9,15,9′-tri-cis- and 9,9′-di-cis-ζ-carotene, while content of downstream carotenoids was significantly decreased. Transcript levels for most of the carotenoid biosynthetic genes showed no alterations in 'Pinalate'; however, the steady-state level mRNA of ζ-carotene isomerase (Z-ISO), which catalyses the conversion of 9,15,9′-tri-cis- to 9,9′-di-cis-ζ-carotene, was significantly reduced both in 'Pinalate' fruit and leaf tissues. Isolation of the 'Pinalate' Z-ISO genomic sequence identified a new allele with a single nucleotide insertion at the second exon, which generates an alternative splicing site that alters Z-ISO transcripts encoding non-functional enzyme. Moreover, functional assays of citrus Z-ISO in E.coli showed that light is able to enhance a non-enzymatic isomerization of tri-cis to di-cis-ζ-carotene, which is in agreement with the partial rescue of mutant phenotype when 'Pinalate' fruits are highly exposed to light during ripening. Conclusion: A single nucleotide insertion has been identified in 'Pinalate' Z-ISO gene that results in truncated proteins. This causes a bottleneck in the carotenoid pathway with an unbalanced content of carotenes upstream to β,β-xanthophylls in fruit tissues. In chloroplastic tissues, the effects of Z-ISO alteration are mainly manifested as a reduction in total carotenoid content. Taken together, our results indicate that the spontaneous single nucleotide insertion in Z-ISO is the molecular basis of the yellow pigmentation in 'Pinalate' sweet orange and points this isomerase as an essential activity for carotenogenesis in citrus fruits.
© 2019 The Author(s). MenosAbstract.
Background: Fruit coloration is one of the main quality parameters of Citrus fruit primarily determined by genetic factors. The fruit of ordinary sweet orange (Citrus sinensis) displays a pleasant orange tint due to accumulation of carotenoids, representing β,β-xanthophylls more than 80% of the total content. 'Pinalate' is a spontaneous bud mutant, or somatic mutation, derived from sweet orange 'Navelate', characterized by yellow fruits due to elevated proportions of upstream carotenes and reduced β,β-xanthophylls, which suggests a biosynthetic blockage at early steps of the carotenoid pathway. Results: To identify the molecular basis of 'Pinalate' yellow fruit, a complete characterization of carotenoids profile together with transcriptional changes in carotenoid biosynthetic genes were performed in mutant and parental fruits during development and ripening. 'Pinalate' fruit showed a distinctive carotenoid profile at all ripening stages, accumulating phytoene, phytofluene and unusual proportions of 9,15,9′-tri-cis- and 9,9′-di-cis-ζ-carotene, while content of downstream carotenoids was significantly decreased. Transcript levels for most of the carotenoid biosynthetic genes showed no alterations in 'Pinalate'; however, the steady-state level mRNA of ζ-carotene isomerase (Z-ISO), which catalyses the conversion of 9,15,9′-tri-cis- to 9,9′-di-cis-ζ-carotene, was significantly reduced both in 'Pinalate' fruit an... Presentar Todo |
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Palabras claves : |
Carotenoid; GENE EXPRESSION; MUTANT; Pigmentation; PLATAFORMA AGROALIMENTOS; RIPENING; Zeta-carotene isomerase. |
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Thesagro : |
CAROTENOIDES; CITRUS. |
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Asunto categoría : |
F01 Cultivo |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/14051/1/BMC-Plant-Pathology-2019-s12870-019-2078-2.pdf
https://bmcplantbiol.biomedcentral.com/track/pdf/10.1186/s12870-019-2078-2
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Marc : |
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001 1060661
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008 2019 bl uuuu u00u1 u #d
022 $a1471-2229
024 7 $a10.1186/s12870-019-2078-2$2DOI
100 1 $aRODRIGO, M.J.
245 $aA mutant allele of ζ-carotene isomerase (Z-ISO) is associated with the yellow pigmentation of the "pinalate" sweet orange mutant and reveals new insights into its role in fruit carotenogenesis.$h[electronic resource]
260 $c2019
500 $aArticle history: Received: 17 June 2019 / Accepted: 16 October 2019 / Published online: 4 November 2019. Funding text: Financial support of the research grants AGL2012?34576 and AGL2015? 70218 (Ministerio Economía y Competitividad, Spain), and RTI2018-095131-BI00 (Ministerio Ciencia, Innovacion y Universidades, Spain). Work in the group of JH was supported by Israel Science Foundation Grant 850/13. MJR, LZ, JH and OD are members of Eurocaroten (COST_Action CA15136). MJR and LZ belong to CaRed (Spanish Carotenoid Network BIO2017?90877-REDT, Minis-terio de Ciencia, Innovación y Universidades, Spain). Authors acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI). Supplementary information accompanies this paper.
520 $aAbstract. Background: Fruit coloration is one of the main quality parameters of Citrus fruit primarily determined by genetic factors. The fruit of ordinary sweet orange (Citrus sinensis) displays a pleasant orange tint due to accumulation of carotenoids, representing β,β-xanthophylls more than 80% of the total content. 'Pinalate' is a spontaneous bud mutant, or somatic mutation, derived from sweet orange 'Navelate', characterized by yellow fruits due to elevated proportions of upstream carotenes and reduced β,β-xanthophylls, which suggests a biosynthetic blockage at early steps of the carotenoid pathway. Results: To identify the molecular basis of 'Pinalate' yellow fruit, a complete characterization of carotenoids profile together with transcriptional changes in carotenoid biosynthetic genes were performed in mutant and parental fruits during development and ripening. 'Pinalate' fruit showed a distinctive carotenoid profile at all ripening stages, accumulating phytoene, phytofluene and unusual proportions of 9,15,9′-tri-cis- and 9,9′-di-cis-ζ-carotene, while content of downstream carotenoids was significantly decreased. Transcript levels for most of the carotenoid biosynthetic genes showed no alterations in 'Pinalate'; however, the steady-state level mRNA of ζ-carotene isomerase (Z-ISO), which catalyses the conversion of 9,15,9′-tri-cis- to 9,9′-di-cis-ζ-carotene, was significantly reduced both in 'Pinalate' fruit and leaf tissues. Isolation of the 'Pinalate' Z-ISO genomic sequence identified a new allele with a single nucleotide insertion at the second exon, which generates an alternative splicing site that alters Z-ISO transcripts encoding non-functional enzyme. Moreover, functional assays of citrus Z-ISO in E.coli showed that light is able to enhance a non-enzymatic isomerization of tri-cis to di-cis-ζ-carotene, which is in agreement with the partial rescue of mutant phenotype when 'Pinalate' fruits are highly exposed to light during ripening. Conclusion: A single nucleotide insertion has been identified in 'Pinalate' Z-ISO gene that results in truncated proteins. This causes a bottleneck in the carotenoid pathway with an unbalanced content of carotenes upstream to β,β-xanthophylls in fruit tissues. In chloroplastic tissues, the effects of Z-ISO alteration are mainly manifested as a reduction in total carotenoid content. Taken together, our results indicate that the spontaneous single nucleotide insertion in Z-ISO is the molecular basis of the yellow pigmentation in 'Pinalate' sweet orange and points this isomerase as an essential activity for carotenogenesis in citrus fruits. © 2019 The Author(s).
650 $aCAROTENOIDES
650 $aCITRUS
653 $aCarotenoid
653 $aGENE EXPRESSION
653 $aMUTANT
653 $aPigmentation
653 $aPLATAFORMA AGROALIMENTOS
653 $aRIPENING
653 $aZeta-carotene isomerase
700 1 $aLADO, J.
700 1 $aALÓS, E.
700 1 $aALQUÉZAR, B.
700 1 $aDERY, O.
700 1 $aHIRSCHBERG, J.
700 1 $aZACARÍAS, L.
773 $tBMC Plant Biology, 4 November 2019, Volume 19, Issue 1, Article number 465. OPEN ACCESS. Doi: DOI: 10.1186/s12870-019-2078-2
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela; INIA Treinta y Tres. |
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Fecha actual : |
27/09/2017 |
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Actualizado : |
25/10/2019 |
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Tipo de producción científica : |
Documentos |
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Autor : |
(INIA) INSTITUTO NACIONAL DE INVESTIGACIÓN AGROPECUARIA, URUGUAY; (INASE) INSTITUTO NACIONAL DE SEMILLAS, URUGUAY. |
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Título : |
Resultados experimentales de la evaluación nacional de cultivares de trigo ciclo largo: período 2016. |
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Fecha de publicación : |
2017 |
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Fuente / Imprenta : |
La Estanzuela (UY): INASE ; INIA, 2017. |
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Páginas : |
31 p. |
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Idioma : |
Español |
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Notas : |
Editado por el Equipo de Evaluación de Cultivares - Impreso por Unidad de Comunicación y Transferencia de Tecnología INIA La Estanzuela.
Convenio INASE-INIA. |
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Contenido : |
EQUIPOS DE TRABAJO
INIA
Evaluación de Cultivares
Ing. Agr. (PhD.) Marina Castro
Evaluación de Cultivares
Ing. Agr. Santiago Manasliski
Ensayos regionales Young
Téc. Sist. Int. Gan. Máximo Vera
Asistente de Investigación
Beatriz Castro
Valeria Cardozo
Asistentes de Información y Proc. de datos
Protección Vegetal
Ing. Agr. (Ph.D.) Silvia Pereyra (Fitopatología)
Lic. Biol. (Ph.D.) Gustavo Azzimonti (Fitopatología)
Ing. Agr. (Ph.D.) Silvia Germán (Mej. por resistencia)
Tec. Agrop. Richard García (Mej. por resistencia)
Tec. Lech. Néstor González (Fitopatología)
Unidad de Comunicación y Transferencia de Tecnología
Ing. Agr. (M.Sc.) Ernesto Restaino
Amado Vergara (Asistente de UCTT)
SOCIEDAD RURAL DE RÍO NEGRO
Ing. Agr. (MBA) Donald Chalkling
Téc. Agrop. Santiago Salaberry
Martha Roth
INASE
Área Evaluación y Registro de Cultivares
Ing. Agr. (M.Sc.) Gerardo Camps
Gerente
Ing. Agr. (M.Sc.) Virginia Olivieri
Ing. Agr. (M.Sc.) Sebastián Moure
Ing. Agr. (M.Sc.) Federico Boschi
Área Laboratorio de Calidad de Semillas
Ph.D. Vanessa Sosa
Gerente
Ing. Agr. Teresita Farrás
Analista Fabián Makowski
Analista Mónica Rojas
Analista Laura Tellechea
Área Administración
Daniel Almeida.
TABLA DE CONTENIDO.I. PRESENTACIÓN II. CARACTERIZACIÓN DE LA ZAFRA 2016. III. EVALUACION DE CULTIVARES DE TRIGO CICLO LARGO.
1. INTRODUCCION 2. OBJETIVO.3. MATERIALES Y METODOS. 3.1 Ensayos conducidos en La Estanzuela y Young. 3.2 Ensayos conducidos en Dolores.
4. RESULTADOS EXPERIMENTALES ? Ensayos sin fungicidas.4.1 Rendimiento de grano.4.2 Comportamiento sanitario.
4.2.1 Comportamiento sanitario en colecciones.4.3 Características agronómicas.4.4 Calidad de grano.
5. RESULTADOS EXPERIMENTALES ? Ensayos con fungicidas.5.1 Rendimiento de grano.5.2 Características agronómicas.
5.3 Calidad de grano.IV. CONDICIONES CLIMATICAS. MenosEQUIPOS DE TRABAJO
INIA
Evaluación de Cultivares
Ing. Agr. (PhD.) Marina Castro
Evaluación de Cultivares
Ing. Agr. Santiago Manasliski
Ensayos regionales Young
Téc. Sist. Int. Gan. Máximo Vera
Asistente de Investigación
Beatriz Castro
Valeria Cardozo
Asistentes de Información y Proc. de datos
Protección Vegetal
Ing. Agr. (Ph.D.) Silvia Pereyra (Fitopatología)
Lic. Biol. (Ph.D.) Gustavo Azzimonti (Fitopatología)
Ing. Agr. (Ph.D.) Silvia Germán (Mej. por resistencia)
Tec. Agrop. Richard García (Mej. por resistencia)
Tec. Lech. Néstor González (Fitopatología)
Unidad de Comunicación y Transferencia de Tecnología
Ing. Agr. (M.Sc.) Ernesto Restaino
Amado Vergara (Asistente de UCTT)
SOCIEDAD RURAL DE RÍO NEGRO
Ing. Agr. (MBA) Donald Chalkling
Téc. Agrop. Santiago Salaberry
Martha Roth
INASE
Área Evaluación y Registro de Cultivares
Ing. Agr. (M.Sc.) Gerardo Camps
Gerente
Ing. Agr. (M.Sc.) Virginia Olivieri
Ing. Agr. (M.Sc.) Sebastián Moure
Ing. Agr. (M.Sc.) Federico Boschi
Área Laboratorio de Calidad de Semillas
Ph.D. Vanessa Sosa
Gerente
Ing. Agr. Teresita Farrás
Analista Fabián Makowski
Analista Mónica Rojas
Analista Laura Tellechea
Área Administración
Daniel Almeida.
TABLA DE CONTENIDO.I. PRESENTACIÓN II. CARACTERIZACIÓN DE LA ZAFRA 2016. III. EVALUACION DE CULTIVARES DE TRIGO CICLO LARGO.
1. INTRODUCCION 2. OBJETIVO.3. MATERIALES Y METODOS. 3.1 Ensayos conducidos en La Estanzuela y Young. 3.2 Ensayos conducidos en Dolores.
4. RESULTADOS EXPERIMENTALES ? Ensayos sin fungicidas.4... Presentar Todo |
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Thesagro : |
EVALUACION DE CULTIVARES; TRIGO. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/9394/1/PubTrigoLargoPeriodo2016.pdf
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Marc : |
LEADER 02592nam a2200169 a 4500
001 1057613
005 2019-10-25
008 2017 bl uuuu u0uu1 u #d
100 1 $a(INIA) INSTITUTO NACIONAL DE INVESTIGACIÓN AGROPECUARIA, URUGUAY
245 $aResultados experimentales de la evaluación nacional de cultivares de trigo ciclo largo$bperíodo 2016.$h[electronic resource]
260 $aLa Estanzuela (UY): INASE ; INIA$c2017
300 $a31 p.
500 $aEditado por el Equipo de Evaluación de Cultivares - Impreso por Unidad de Comunicación y Transferencia de Tecnología INIA La Estanzuela. Convenio INASE-INIA.
520 $aEQUIPOS DE TRABAJO INIA Evaluación de Cultivares Ing. Agr. (PhD.) Marina Castro Evaluación de Cultivares Ing. Agr. Santiago Manasliski Ensayos regionales Young Téc. Sist. Int. Gan. Máximo Vera Asistente de Investigación Beatriz Castro Valeria Cardozo Asistentes de Información y Proc. de datos Protección Vegetal Ing. Agr. (Ph.D.) Silvia Pereyra (Fitopatología) Lic. Biol. (Ph.D.) Gustavo Azzimonti (Fitopatología) Ing. Agr. (Ph.D.) Silvia Germán (Mej. por resistencia) Tec. Agrop. Richard García (Mej. por resistencia) Tec. Lech. Néstor González (Fitopatología) Unidad de Comunicación y Transferencia de Tecnología Ing. Agr. (M.Sc.) Ernesto Restaino Amado Vergara (Asistente de UCTT) SOCIEDAD RURAL DE RÍO NEGRO Ing. Agr. (MBA) Donald Chalkling Téc. Agrop. Santiago Salaberry Martha Roth INASE Área Evaluación y Registro de Cultivares Ing. Agr. (M.Sc.) Gerardo Camps Gerente Ing. Agr. (M.Sc.) Virginia Olivieri Ing. Agr. (M.Sc.) Sebastián Moure Ing. Agr. (M.Sc.) Federico Boschi Área Laboratorio de Calidad de Semillas Ph.D. Vanessa Sosa Gerente Ing. Agr. Teresita Farrás Analista Fabián Makowski Analista Mónica Rojas Analista Laura Tellechea Área Administración Daniel Almeida. TABLA DE CONTENIDO.I. PRESENTACIÓN II. CARACTERIZACIÓN DE LA ZAFRA 2016. III. EVALUACION DE CULTIVARES DE TRIGO CICLO LARGO. 1. INTRODUCCION 2. OBJETIVO.3. MATERIALES Y METODOS. 3.1 Ensayos conducidos en La Estanzuela y Young. 3.2 Ensayos conducidos en Dolores. 4. RESULTADOS EXPERIMENTALES ? Ensayos sin fungicidas.4.1 Rendimiento de grano.4.2 Comportamiento sanitario. 4.2.1 Comportamiento sanitario en colecciones.4.3 Características agronómicas.4.4 Calidad de grano. 5. RESULTADOS EXPERIMENTALES ? Ensayos con fungicidas.5.1 Rendimiento de grano.5.2 Características agronómicas. 5.3 Calidad de grano.IV. CONDICIONES CLIMATICAS.
650 $aEVALUACION DE CULTIVARES
650 $aTRIGO
700 1 $a(INASE) INSTITUTO NACIONAL DE SEMILLAS, URUGUAY.
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